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mesenchymal stem cell adipocyte differentiation medium  (PromoCell)


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    PromoCell mesenchymal stem cell adipocyte differentiation medium
    Single-cell RNA sequencing profiling of joint cell populations following extracellular vesicle (EVs) treatment. (a) The UMAP plot displaying the classification of joint cell populations into ten distinct clusters under three conditions: WT, PBS-treated (PBS), and EVs-treated (EVs). ( b ) Cell typing of clusters based on gene expression. Cluster 1 (NK cells), Cluster 2 (T cells), Cluster 3 (pre-B cells), Cluster 4 (Neutrophils), Cluster 5 (B cells), Cluster 6 (Chondrocyte progenitor cells), Cluster 7 (Hematopoietic progenitors), Cluster 8 <t>(Mesenchymal</t> cells), Cluster 9 (Plasma cells), and Cluster 10 (Erythroblasts). ( c ) Heatmap displaying the expression of representative marker genes for each cluster, including Col12a1 for the
    Mesenchymal Stem Cell Adipocyte Differentiation Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mesenchymal stem cell adipocyte differentiation medium/product/PromoCell
    Average 97 stars, based on 306 article reviews
    mesenchymal stem cell adipocyte differentiation medium - by Bioz Stars, 2026-02
    97/100 stars

    Images

    1) Product Images from "Extracellular vesicles derived from adipose-derived mesenchymal stem/stromal cells prevent synovial inflammation and attenuate cartilage degeneration in rodent osteoarthritis"

    Article Title: Extracellular vesicles derived from adipose-derived mesenchymal stem/stromal cells prevent synovial inflammation and attenuate cartilage degeneration in rodent osteoarthritis

    Journal: Regenerative Therapy

    doi: 10.1016/j.reth.2025.101056

    Single-cell RNA sequencing profiling of joint cell populations following extracellular vesicle (EVs) treatment. (a) The UMAP plot displaying the classification of joint cell populations into ten distinct clusters under three conditions: WT, PBS-treated (PBS), and EVs-treated (EVs). ( b ) Cell typing of clusters based on gene expression. Cluster 1 (NK cells), Cluster 2 (T cells), Cluster 3 (pre-B cells), Cluster 4 (Neutrophils), Cluster 5 (B cells), Cluster 6 (Chondrocyte progenitor cells), Cluster 7 (Hematopoietic progenitors), Cluster 8 (Mesenchymal cells), Cluster 9 (Plasma cells), and Cluster 10 (Erythroblasts). ( c ) Heatmap displaying the expression of representative marker genes for each cluster, including Col12a1 for the
    Figure Legend Snippet: Single-cell RNA sequencing profiling of joint cell populations following extracellular vesicle (EVs) treatment. (a) The UMAP plot displaying the classification of joint cell populations into ten distinct clusters under three conditions: WT, PBS-treated (PBS), and EVs-treated (EVs). ( b ) Cell typing of clusters based on gene expression. Cluster 1 (NK cells), Cluster 2 (T cells), Cluster 3 (pre-B cells), Cluster 4 (Neutrophils), Cluster 5 (B cells), Cluster 6 (Chondrocyte progenitor cells), Cluster 7 (Hematopoietic progenitors), Cluster 8 (Mesenchymal cells), Cluster 9 (Plasma cells), and Cluster 10 (Erythroblasts). ( c ) Heatmap displaying the expression of representative marker genes for each cluster, including Col12a1 for the "Chondrocyte" cluster, and Col1a1 for the "Mesenchymal cell" cluster, and CD45/CD14 for the "Hematopoietic" cluster.

    Techniques Used: RNA Sequencing, Gene Expression, Clinical Proteomics, Expressing, Marker

    Differential gene expression besed on Single-cell RNA sequencing of joint cell clusters following extracellular vesicle (EVs) treatment . ( a-d ) Comparison of gene expression in EVs, PBS, and WT groups (a) in Cluster 6 (chondrocyte progenitors) (COL2A1, COL1A2, PRG4, MMP3, CCL2, and FGF18). ( b ) in Cluster 1 (NK cells) (CD14, MRC1, CD163, CD86, CD80, and NOS2). ( c ) in Cluster 8 (Mesenchymal cells) (COL2A1, COL1A2, PRG4, CCL2, and FGF18) ( d ) in Cluster 4 (Neutrophils) (APOE, AGPAT4, HAPLN1, CDKN1C, MET, and CEMIP2). (e) Flow cytometry analysis confirmed that the proportion of CD11b + CD163 + M2 macrophages was approximately 2.5-fold higher in the EVs-treated group compared to the PBS-treated group.
    Figure Legend Snippet: Differential gene expression besed on Single-cell RNA sequencing of joint cell clusters following extracellular vesicle (EVs) treatment . ( a-d ) Comparison of gene expression in EVs, PBS, and WT groups (a) in Cluster 6 (chondrocyte progenitors) (COL2A1, COL1A2, PRG4, MMP3, CCL2, and FGF18). ( b ) in Cluster 1 (NK cells) (CD14, MRC1, CD163, CD86, CD80, and NOS2). ( c ) in Cluster 8 (Mesenchymal cells) (COL2A1, COL1A2, PRG4, CCL2, and FGF18) ( d ) in Cluster 4 (Neutrophils) (APOE, AGPAT4, HAPLN1, CDKN1C, MET, and CEMIP2). (e) Flow cytometry analysis confirmed that the proportion of CD11b + CD163 + M2 macrophages was approximately 2.5-fold higher in the EVs-treated group compared to the PBS-treated group.

    Techniques Used: Gene Expression, RNA Sequencing, Comparison, Flow Cytometry



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    Image Search Results


    Single-cell RNA sequencing profiling of joint cell populations following extracellular vesicle (EVs) treatment. (a) The UMAP plot displaying the classification of joint cell populations into ten distinct clusters under three conditions: WT, PBS-treated (PBS), and EVs-treated (EVs). ( b ) Cell typing of clusters based on gene expression. Cluster 1 (NK cells), Cluster 2 (T cells), Cluster 3 (pre-B cells), Cluster 4 (Neutrophils), Cluster 5 (B cells), Cluster 6 (Chondrocyte progenitor cells), Cluster 7 (Hematopoietic progenitors), Cluster 8 (Mesenchymal cells), Cluster 9 (Plasma cells), and Cluster 10 (Erythroblasts). ( c ) Heatmap displaying the expression of representative marker genes for each cluster, including Col12a1 for the

    Journal: Regenerative Therapy

    Article Title: Extracellular vesicles derived from adipose-derived mesenchymal stem/stromal cells prevent synovial inflammation and attenuate cartilage degeneration in rodent osteoarthritis

    doi: 10.1016/j.reth.2025.101056

    Figure Lengend Snippet: Single-cell RNA sequencing profiling of joint cell populations following extracellular vesicle (EVs) treatment. (a) The UMAP plot displaying the classification of joint cell populations into ten distinct clusters under three conditions: WT, PBS-treated (PBS), and EVs-treated (EVs). ( b ) Cell typing of clusters based on gene expression. Cluster 1 (NK cells), Cluster 2 (T cells), Cluster 3 (pre-B cells), Cluster 4 (Neutrophils), Cluster 5 (B cells), Cluster 6 (Chondrocyte progenitor cells), Cluster 7 (Hematopoietic progenitors), Cluster 8 (Mesenchymal cells), Cluster 9 (Plasma cells), and Cluster 10 (Erythroblasts). ( c ) Heatmap displaying the expression of representative marker genes for each cluster, including Col12a1 for the "Chondrocyte" cluster, and Col1a1 for the "Mesenchymal cell" cluster, and CD45/CD14 for the "Hematopoietic" cluster.

    Article Snippet: For adipocyte differentiation, either BioMirai Lab's induction kit or PromoCell's mesenchymal stem cell adipocyte differentiation medium was used, with Oil Red O staining to assess lipid accumulation.

    Techniques: RNA Sequencing, Gene Expression, Clinical Proteomics, Expressing, Marker

    Differential gene expression besed on Single-cell RNA sequencing of joint cell clusters following extracellular vesicle (EVs) treatment . ( a-d ) Comparison of gene expression in EVs, PBS, and WT groups (a) in Cluster 6 (chondrocyte progenitors) (COL2A1, COL1A2, PRG4, MMP3, CCL2, and FGF18). ( b ) in Cluster 1 (NK cells) (CD14, MRC1, CD163, CD86, CD80, and NOS2). ( c ) in Cluster 8 (Mesenchymal cells) (COL2A1, COL1A2, PRG4, CCL2, and FGF18) ( d ) in Cluster 4 (Neutrophils) (APOE, AGPAT4, HAPLN1, CDKN1C, MET, and CEMIP2). (e) Flow cytometry analysis confirmed that the proportion of CD11b + CD163 + M2 macrophages was approximately 2.5-fold higher in the EVs-treated group compared to the PBS-treated group.

    Journal: Regenerative Therapy

    Article Title: Extracellular vesicles derived from adipose-derived mesenchymal stem/stromal cells prevent synovial inflammation and attenuate cartilage degeneration in rodent osteoarthritis

    doi: 10.1016/j.reth.2025.101056

    Figure Lengend Snippet: Differential gene expression besed on Single-cell RNA sequencing of joint cell clusters following extracellular vesicle (EVs) treatment . ( a-d ) Comparison of gene expression in EVs, PBS, and WT groups (a) in Cluster 6 (chondrocyte progenitors) (COL2A1, COL1A2, PRG4, MMP3, CCL2, and FGF18). ( b ) in Cluster 1 (NK cells) (CD14, MRC1, CD163, CD86, CD80, and NOS2). ( c ) in Cluster 8 (Mesenchymal cells) (COL2A1, COL1A2, PRG4, CCL2, and FGF18) ( d ) in Cluster 4 (Neutrophils) (APOE, AGPAT4, HAPLN1, CDKN1C, MET, and CEMIP2). (e) Flow cytometry analysis confirmed that the proportion of CD11b + CD163 + M2 macrophages was approximately 2.5-fold higher in the EVs-treated group compared to the PBS-treated group.

    Article Snippet: For adipocyte differentiation, either BioMirai Lab's induction kit or PromoCell's mesenchymal stem cell adipocyte differentiation medium was used, with Oil Red O staining to assess lipid accumulation.

    Techniques: Gene Expression, RNA Sequencing, Comparison, Flow Cytometry

    Characterization of mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) used in this study. (A) Canine MSCs at P2 displayed spindle morphology. (B) ALP staining and (C) Alizarin Red S staining verify osteogenic differentiation of MSCs after three weeks of induction. (D) The adipogenic differentiation of MSCs was verified by positive Oil Red O staining after 4 weeks of mass culture and induction. (E) MSCs also had the capacity to undergo chondrogenic differentiation. This was evident through Alcian blue staining after induction for 4 weeks. (F) Flow cytometry analysis of the expression of indicated cell surface markers related to MSCs. (G) Morphology of EPCs at 12 days of culture. EPCs could take up (H) FITC-UEA-1 and bind to (I) Dil-Ac-LDL as shown by the green and red fluorescence. (J) Merged images show that most cells were dual-positive. Dual-positive cells were defined as EPCs. (K) Flow cytometry analysis of the expression of indicated cell surface markers related to EPCs. Scale bar = 100 μm.

    Journal: American Journal of Translational Research

    Article Title: Endothelial progenitor cells with stem cells enhance osteogenic efficacy

    doi:

    Figure Lengend Snippet: Characterization of mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) used in this study. (A) Canine MSCs at P2 displayed spindle morphology. (B) ALP staining and (C) Alizarin Red S staining verify osteogenic differentiation of MSCs after three weeks of induction. (D) The adipogenic differentiation of MSCs was verified by positive Oil Red O staining after 4 weeks of mass culture and induction. (E) MSCs also had the capacity to undergo chondrogenic differentiation. This was evident through Alcian blue staining after induction for 4 weeks. (F) Flow cytometry analysis of the expression of indicated cell surface markers related to MSCs. (G) Morphology of EPCs at 12 days of culture. EPCs could take up (H) FITC-UEA-1 and bind to (I) Dil-Ac-LDL as shown by the green and red fluorescence. (J) Merged images show that most cells were dual-positive. Dual-positive cells were defined as EPCs. (K) Flow cytometry analysis of the expression of indicated cell surface markers related to EPCs. Scale bar = 100 μm.

    Article Snippet: After 3-5 day, the medium was changed to dog mesenchymal stem cell adipocyte differentiation medium (Cyagen Biosciences, Santa Clara, USA).

    Techniques: Staining, Flow Cytometry, Expressing, Fluorescence